The method was developed by team around Ali Ertürk and Hans-Ulrich Dodt …
melanogaster. In DBE samples are stored and imaged as well.  As result the light is scattered on its path through the tissue, which leads to decrease in resolution or even to disappearance of signal in samples thicker than a few tens of micrometers. Explore releases from the Disco 3 label. For dance club gurus, band completists, and anyone who wonders what the Pet Shop Boys would sound like if they were New Order fanatics, Disco 3 is essential; others might do best to stick with Release. This preprocess overcome two drawbacks of antibody labeling of large samples. Listen Now with Amazon Music : DISCO3+ "Please retry" Amazon Music Unlimited: Structures in transparent tissues can be examined by fluorescence microscopy without need for time-consuming physical sectioning and following reconstruction in silico. 3DISCO is histological method which make biological samples more transparent, by using series of organic solvents for matching refractive index of tissue and surrounding medium. , Whole process is relatively easy to perform and require just changes of given solutions with no need for some customized laboratory equipment.
 Main drawback of this solution is bleaching of GFP signal and insufficient clearing of highly myelinated tissue of adult animals. June 16, 2017 5.0 out of 5 stars 1 rating.  After dehydration the sample is rinsed first in dichlormethane (DCM) and finally in dibenzyl ether (DBE) to match the refractive index of tissue and surrounding medium leading to transparent sample. Authors used this solution to clear mouse brain, mouse embryos and whole body of D. , "Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury", "Shrinkage-mediated imaging of entire organs and organisms using uDISCO", "Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal", "Enriched environment reduces glioma growth through immune and non-immune mechanisms in mice", "TGF-β promotes heterogeneity and drug resistance in squamous cell carcinoma", "iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging", "Tridimensional Visualization and Analysis of Early Human Development", "A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue", "Clearing of fixed tissue: a review from a microscopist's perspective", "Chemical Clearing and Dehydration of GFP Expressing Mouse Brains", "Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods", "Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion", "RetroDISCO: Clearing technique to improve quantification of retrograde labeled motor neurons of intact mouse spinal cords", "Seeing Through to a Mouse's Nervous System", "New Technique Shrinks, Makes Small Animals Transparent", "A 'revolutionary' new technology can turn mice clear — and might one day map your brain", "Scientists can see through these rodents", "2016 in pictures: The best science images of the year", "A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System", "Three-Dimensional Study of Alzheimer's Disease Hallmarks Using the iDISCO Clearing Method", https://en.wikipedia.org/w/index.php?title=3DISCO&oldid=950836926, Creative Commons Attribution-ShareAlike License, initial dehydration performed with tetrahydrofuran (THF), following extraction of lipids via incubation in dichlormethane (DCM), immersion in dibenzyl ether (DBE) for RI matching, This page was last edited on 14 April 2020, at 02:57.
In recent years the use of “DISCO” methods is broadened to research on many other tissues, including single-cell mapping of transplanted stem cells in whole mouse organs, imaging of whole human embryos in different developmental stages or examination and diagnostics of human tumors tissue. , The biological samples (tissues) are heterogeneous structures consist of compounds which differ in their refractive indexes. The method was developed by team around Ali Ertürk and Hans-Ulrich Dodt from Max-Planck-Institute in Munich primarily for clearing and imaging unsectioned mouse brain and spinal cord. View credits, reviews, tracks and shop for the 2003 CD release of Disco 3 on Discogs. Where the Streets Have No Name (I Can't Take My Eyes Off You), I Wouldn't Normally Do This Kind of Thing, Se a vida é (That's the Way Life Is)"/"To Step Aside, I Don't Know What You Want but I Can't Give It Any More, You Only Tell Me You Love Me When You're Drunk, https://en.wikipedia.org/w/index.php?title=Disco_3&oldid=973767318, Short description is different from Wikidata, Album articles lacking alt text for covers, Articles with album ratings that need to be turned into prose, Album chart usages for BillboardDanceElectronic, Creative Commons Attribution-ShareAlike License, "Try It (I'm in Love with a Married Man)", "Positive Role Model" includes a sample of ", Andy Kaufhold – remix, additional production, This page was last edited on 19 August 2020, at 03:13. DISCO3+ HEALTH. "Positive Role Model" also appeared on the Germany-… It was also chosen as one of the top 10 scientific images of 2016 by Nature.  3DISCO was also used for mapping the development of mouse CNS. Clearing methods, including 3DISCO, was mainly developed for neuroscience research first. Note that this chapter illustrates development and use of solvent-based clearing methods and does not provide complete list of applications and modifications of them. See all 3 formats and editions Hide other formats and editions. Their renaissance came in last decade, probably thank to spread of advanced techniques of fluorescence microscopy which allow optical sectioning of the specimen (confocal, multiphoton or light sheet microscopy).
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